Arbeitsgruppe "Ancient Proteins" Dr. Shevan Wilkin
In the intricate mosaic of archaeological research, proteins provide a view into the past, offering a unique perspective on ancient cultures and practices. The burgeoning field of archaeological proteins, sometimes referred to as palaeoproteomics, has revolutionized our understanding of history by providing insights into human-animal interactions, diet, and ancient health.
Through meticulous extraction and analysis techniques, archaeologists can recover proteins from a variety of archaeological materials, including bones, teeth, textiles, and organic residues. These ancient biomolecules, often preserved over millennia in burial sites, settlements, or on ancient artifacts, unlock a trove of information about past societies and their interactions with the environment.
From the identification of dietary staples through the analysis of ancient proteins in dental calculus to the reconstruction of ancient trade networks using protein residues from vessels, the study of archaeological proteins offers a multidisciplinary approach to unraveling the complexities of human history. Furthermore, the integration of archaeological proteins with other scientific disciplines, such as isotopic analysis, DNA sequencing, and microscopy, enhances our ability to reconstruct past lifeways with unprecedented detail and accuracy. As we delve deeper into the realm of archaeological proteins, we embark on a journey through time, piecing together the fragments of the past to illuminate the rich tapestry of human experience and heritage.
Introductory literature:
- Hendy, J. Ancient protein analysis in archaeology. Sci Adv 7, (2021).
- Wilkin, S., Miller, A. V., Boivin, N., Pedergnana, A., Shishlina, N., Trifonov, V. & Hommel, A. P. Curated cauldrons: Preserved proteins from early copper alloy vessels illuminate feasting practices in the Caucasian steppe. iScience
- Wilkin, S., Miller, A. V., Taylor, W., Miller, B. K., Hagan, R., Bleasdale, M., Scott, A., Gankhuyag, S., Ramsøe, A., Ulziibayar, S., Trachsel, C., Nanni, P., Grossmann, J., Orlando, L., Horton, M., Stockhammer, P., Myagmar, E., Boivin, N., Warinner, C. & Hendy}, J. Dairy pastoralism sustained Eastern Eurasian steppe populations for 5000 years. Nature Ecology and Evolution (2020).
The Ambizione grant expands on a previous paper of this group combining ancient protein and DNA analysis to better understand the human immune response to mycobacterial diseases (tuberculosis, leprosy) in the past.
Using a multi-enzyme protocol, we deeply explore the human proteome, focusing on those with immune-related functions, to pinpoint whether there are distinct immune profiles or specific biomarkers present in archaeological skeletal remains. Protein analysis is done in tandem with pathogen DNA analysis of the same archaeological individuals to recover pathogen genomes from Mycobacterium leprae and M. tuberculosis.
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For more information on sample preparation and protein extraction, please refer to our guides / protocols listed below.
Sampling from curation collections or from the field
Sample preparation in the lab
Data analysis